Fluorimetric Immunoassay for Multianalysis of Aflatoxins
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چکیده
منابع مشابه
Fluorimetric Immunoassay for Multianalysis of Aflatoxins
A sensitive fluorimetric ELISA was developed for the analysis of aflatoxins. The assay was performed in a 384 microwell plate, wherein high specificity monoclonal antibody against AFM1 (mAb-AFM1) was used as capture antibody and FITC conjugated secondary antibody was used for detection and quantification of the analyte. The linear range of the immunoassay was found to be 6.25-50 pg/mL. AFM1 as ...
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A systematic spectrofluorimetric study of the formation of ternary complexes of terbium ions (Tb3’) with ethylenediamine tetraacetic acid (EDTA) and a variety of bidentate organic ligands has been performed. Fourteen ligands (salicylic acid, quinolones and hydroxybenzene sulfonic acid derivatives) were examined as efficient energy donating chelators for Tb3+. The fluorescence properties of the ...
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An immunoassay-based lateral flow device for the quantitative determination of four major aflatoxins in maize has been developed. The one-step assay has performance comparably with that of other screening methods, as confirmed by the intra- and the inter-day precision of the data (RSD 10-22%), and can be completed in 10 min. Quantification was obtained by acquiring images of the strip and corre...
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(7) L’vov, B. V. Spectrochim. Acfa, Pafl B 1078, 338, 153-193. (8) Hageman, L. R.; Nlchols, J. A.; Viswanedham, P.; Woodriff, R. Anal. Chem. 1070, 51, 1406-1412. (9) Chakrabartt, C. L.; Wain, C. C.; Hamed, H. A.; Bartels, P. C. Anal. Chem. 1081, 53, 444-450. (IO) Barnard, T. Anal. Chern. 1070, 51, 1172A-1178A. (11) Siemer, D. D.; Baldwln, J. M. Anal. Chem. 1080, 52, 295-300. (12) L’vov, B. V. “...
متن کاملBiosensors for Aflatoxins Detection
The availability of rapid and reliable methods for rapid determination of small molecules, as contaminants in food samples, including Aflatoxins, is an increasing need also for human health. In order to monitoring food contaminants, as Mycotoxins (MTXs) Gas Chromatographic (GC) and High Pressure Liquid Chromatography (HPLC) methods are generally utilized, due to their high detection sensibility...
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ژورنال
عنوان ژورنال: Journal of Analytical Methods in Chemistry
سال: 2013
ISSN: 2090-8865,2090-8873
DOI: 10.1155/2013/584964